SNPs Z2103 and Z2105 have been available for the Sanger sequencing test from FTDNA for awhile. YSEQ recently added Z2104, Z2107 and Z2110/CTS7822 to their catalog of SNPs that can be tested. So I took the plunge and ordered all three from YSEQ. My goal is to have quality Sanger sequencing data for Z2103 through Z2110. Data that could be used for the ISOGG haplogroup tree if needed.
Z2110/CTS7822 came in negative in just two weeks. YSEQ is good and quick.
Z2104 is presenting some challenges because two sections of my genome are competing for attention. This SNP is in a homologous region that can recombine with a different chromosome. Z2104 YSEQ problem bhdiijah.png The double peak is highlighted in blue. Dr.Krahn in trying "Nested PCR" for additional filtering that may or may not work. We will know soon. Thanks to Thomas for this information and the extra effort.
I was caught mentally flat footed by this.
Fortunately Vince T had a post on another thread the other day that gave some clues on what this might mean for picking SNPs to test.
What are the good regions of the Y chromosome for Sanger sequencing? How far from the Y-centromere should the SNP be?
Based on distance from the Y-centromere, are Z2104, Z2106, Z2108 and Z2109 in a more difficult region for Sanger sequencing? Conversely, are Z2103, Z2105, Z2107 and Z2110 in more suitable regions? Attached are the ISOGG YBrowse browser views of Z2103 thru Z2110 for comparison.
Z-SNPs-Y-Browse.jpg or large view.
Z2104 ISOGG YBrowse